Abridged Bibliography of Studies on Collagen Peptides

The benefits of collagen peptides for health and beauty have been confirmed and recognized for years. Scientific studies have shown that 10 grams a day or even less have a positive effect on joint, bone and skin health.

Collagen peptides have been subject to scientific research and have been shown to be free of adverse side effects. It can simply be integrated into the conventional manufacturing process of the respective product.

To the best knowledge of GROW, a list of scientific studies on benefits of collagen peptides can be found below.

Scientific Studies on Collagen Peptides

Joint & Bone Health

(1) Dubourg,G.,(2013).Targeting bone health. The world of food ingredients, February 2013.

(2) Kim, H.K., Kim, M.G. and Leem, K.H. (2013). Osteogenic activity of collagen peptide via ERK/MAPK pathway mediated boosting of collagen synthesis and its therapeutic efficacy in osteoporotic bone by back-scattered electron imaging and microarchitecture analysis. Molecules, Vol. 18, 15474-15489.

(3) Leem, K.H., Lee, S., Jang, A. and Kim, H.K. (2013). Porcine skin gelatin hydrolysate promotes longitudinal bone growth in adolescent rats. Journal of Medicinal Food, Vol. 16, Iss. 5, 447-453.

(4) Kim, H.K., Kim, M.G. and Leem, K.H. (2014). Collagen hydrolysates increased osteogenic gene expressions via a MAPK signaling pathway in MG-63 human osteoblasts. Food & Function, Vol. 5, Iss. 3, 573-578.

(5) JunLi Liu, Bing Zhang, ShuJun Song, Ming Ma, ShaoYan Si, YiHu Wang, BingXin Xu, Kai Feng, JiGong Wu and YanChuan Guo. (2014). Bovine collagen peptides compounds promote the proliferation and differentiation of MC3T3-E1 pre-osteoblasts, Vol.9, Iss. 6, 1-6.

(6) Adam, M., Spacek, P., Hulejova, H., Galianova, A. and Blahos, J. (1996). Postmenopausal osteoporosis. Treatment with calcitonin and a diet rich in collagen peptides. Casopis Lékaru Ceských, Vol. 135, Iss. 3, 74-78.

(7) Martin-Bautista, E., Martin-Matillas, M., Martin-Lagos, J.A., Miranda-Leon, M.T., Muñoz-Torres, M., Ruiz-Requena, E., Rivero, M., Quer, J., Puigdueta, I., and Campoy, C. (2011). A nutritional intervention study with hydrolyzed collagen in pre-pubertal spanish children: influence on bone modeling biomarkers. Journal of Pediatric Endocrinology and Metabolism, Vol. 24, Iss. 3-4,147-153.

(8) Trč, T. and Bohmová, J. (2011). Efficacy and tolerance of enzymatic hydrolysed collagen (EHC) vs. glucosamine sulphate (GS) in the treatment of knee osteoarthritis (KOA). International Orthopaedics, Vol. 35, Iss. 3, 341-348.

(9) Benito-Ruiz, P., Camacho-Zambrano, M.M., Carrillo-Arcentales, J.N., Mestanza-Peralta, M.A., Vallejo-Flores, C.A., Vargas-López, S.V., Villacís-Tamayo, R.A. and Zurita-Gavilanes, L.A. (2009). A randomized controlled trial on the efficacy and safety of a food ingredient, collagen hydrolysate, for improving joint comfort. International Journal of Food Sciences and Nutrition, Vol. 60, Iss. 1, 99-113.

(10) Clark, K.L., Sebastianelli, W., Flechsenhar, K.R., Aukermann, D.F., Meza, F., Millard, R.L., Deitch, J.R., Sherbondy, P.S., and Albert, A. (2008). 24-Week study on the use of collagen hydrolysate as a dietary supplement in athletes with activity-related joint pain. Current Medical Research and Opinions, Vol. 24, Iss. 5, 1485-1496.

(11) Schunck, M., Schulze, C.H. and Oesser, S. (2006). Disparate efficacy of collagen hydrolysate and glucosamine on the extracellular matrix metabolism of articular chondrocytes. Osteoarthritis and Cartilage, Vol. 14, Suppl. 114, 199.

(12) Knox NutraJoint, (2000). New study shows gelatine supplementation may help improve knee function in people with mild osetoarthritis. Partner in motion.

(13) F.Guillerminet, H. Beaupied, V. Fabien-Soulé, D.Tomé, C-L. Benhamou, C. Roux, A. Blais. (2010). Collagen peptides improve bone metabolism and biomechanical parameters in ovariectomized mice: an in vitro and in vivo study. Bone 46 (3): 827-834.

(14) T.E. McAlindon, M. Nuite, N.Krishnan, R. Ruthazer, L.L. Price, D. Bursteien, J. Griffith, K. Flechsenhar. (2011). Change in knee osteoarthritis cartilage detected by delayed gadolinium enhanced magnetic resonance imaging following treatment with collagen hydrolysate: a pilot randomized controlled trial OARSI - ICRS Volume 19, Issue 4.

(15) F.Guillerminet,V. Fabien-Soulé,P.C. Even, D.Tomé, C-L. Benhamou, C. Roux, A. Blais. (2012). Hydrolyzed collagen improves bone status and prevents bone loss in ovariectomized C3H/HeN mice. Osteoporosis International 23 (7): 1909-1919.

(16) M. Schunck, S. Oesser. (2013). Specific collagen peptides benefit the biosynthesis of matrix molecules of tendons and ligaments. Journal of the International Society of Sports Nutrition 10 (Suppl 1).

(17) J.X. Jiang,S. Yu, Q.R. Huang, X.L Zhang, C.Q. Zhang, J.L Zhou, J. Prawitt .(2014). Collagen peptides improve knee osteoarthritis in elderly women. Agro Food Industry Hi Tech 25 (2): 19-23.

(18) Igase M, Kohara K, Okada Y, Ochi M, Igase K, Inoue N, Kutsuna T, Miura H, Ohyagi Y. A double-blind, placebo-controlled, randomised clinical study of the effect of pork collagen peptide supplementation on atherosclerosis in healthy older individuals. Biosci Biotechnol Biochem. (2018) ;82(5):893-895. doi: 10.1080/09168451.2018.1434406. Epub 2018 Feb 15. PMID: 29447076.

(19) Kumar S, Sugihara F, Suzuki K, Inoue N, Venkateswarathirukumara S. A double-blind, placebo-controlled, randomised, clinical study on the effectiveness of collagen peptide on osteoarthritis. J Sci Food Agric. (2015) Mar 15;95(4):702-7. doi: 10.1002/jsfa.6752. Epub 2014 Jun 24. PMID: 24852756.

(20) Soniwala, S. et al. (2018). Oral Hydrolyzed Type 2 Collagen Protects Against the OA of Obesity and Mitigates Obese Gut Microbiome Dysbiosis. Poster presentation at ORS 2018 and OARSI 2018.

(21) Daneault, A. et al. (2014) Hydrolyzed collagen contributes to osteoblast differentiation in vitro and subsequent bone health in vivo. Osteoarthritis and Cartilage, 22:S131.

(22) Wauquier, F. et al. (2019). Human Enriched Serum Following Hydrolysed Collagen Absorption Modulates Bone Cell Activity: from Bedside to Bench and Vice Versa. Nutrients 11: 1249.

(23) Daneault, A. et al., (2017). Biological effect of hydrolyzed collagen on bone metabolism. Critical Reviews in Food Science and Nutrition, 57(9):1922-1937.

(24) Jimi, S., Koizumi, S., Sato, K. et al. (2021) Collagen-derived dipeptide Pro-Hyp administration accelerates muscle regenerative healing accompanied by less scarring after wounding on the abdominal wall in mice. Sci Rep 11, 18750 (2021).

(25) Shiro JIMI, Arman SAPAROV, Seiko KOIZUMI, Motoyasu MIYAZAKI, Satoshi TAKAGI. (2021) A novel mouse wound model for scar tissue formation in abdominal muscle wall. Journal of Veterinary Medical Science, Volume 83, Issue 12, Pages 1933-1942 (2021)

Skin Care

(1) V. Zague. (2008). A new view concerning the effects of collagen hdyrolysate intake on skin properties. Arch Dermatol Res.

(2) V. Zague, V. de Freitas, M. da Cosat Rosa, G. Alvares de Castro, R. G. Jaeger, G. M.Machado-Santelli. (2010). Collagen Hydrolysate Intake Increases Skin Collagen Expression and Suppresses Matrix Mettaloproteinase 2 Activity

(3) Proksch, E., Schunck, M., Zague, V., Segger, D., Degwert, J. and Oesser, S. (2014). Oral intake of specific bioactive collagen peptides reduces skin wrinkles and increases dermal matrix synthesis. Skin Pharmacol. Physiol., Vol 27, Iss. 3, 113-119.

(4) Choi, S.Y., Ko, E.J., Lee, Y.H., Kim, B.G., Shin, H.J., Seo, D.B., Lee, S.J., Kim, B.J. and Kim, M.N. (2013). Effects of collagen tripeptide supplement on skin properties: A prospective, randomized, controlled study. Journal of Cosmetic and Laser Therapy – online ahead of print.

(5) Kantor, I., Donikyan, L.A., Simon, E. And Wollschlaeger, B. (2002). Results of a study evaluating the use of a dietary supplement formula in the management of age-related skin changes in women with moderate to severe wrinkling of the periorbital area. The Journal of the American Nutraceutical Association, Vol. 5, Iss. 2, 10-19.

(6) Proksch, E., Segger, D., Degwert, J., Schunck, M., Zague, V. and Oesser, S. (2014). Oral supplementation of specific collagen peptides has beneficial effects on human skin physiology: a double-blind, placebo-controlled study. Skin Pharmacol. Physiol., Vol. 27, Iss. 1, 47-55.

(7) Ohara, H., Ito, K., Iida, H. And Matsumoto, H. (2009). Improvement in the moisture content of the stratum corneum following 4 weeks of collagen hydrolysate ingestion. Nippon Shokuhin Kagaku Kogaku Kaishi (J. Soc. Food Sci. Technol.) Vol. 56, Iss. 3, 137-145.

(8) Inoue N, Sugihara F, Wang X. Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-blind placebo-controlled clinical study. J Sci Food Agric. (2016);96(12):4077-81. doi: 10.1002/jsfa.7606. Epub 2016 Feb 10. PMID:

(9) Asserin, J. et al. (2015). The effect of oral collagen peptide supplementation on skin moisture and the dermal collagen network: evidence from an ex vivo model and randomized, placebo-controlled clinical trials. Journal of Cosmetic Dermatology, doi: 10.1111/jocd.12174.

(10) Borumand, M. et al., (2014). Daily consumption of the collagen supplement Pure Gold Collagen® reduces visible signs of aging, Clinical Interventions in Aging. Clinical study done by Minerva in UK, 9:1747-1758.

(11) Edgar, S. et al., (2018). Effects of collagen-derived bioactive peptides and natural antioxidant compounds on proliferation and matrix protein synthesis by cultured normal human dermal fibroblasts. Clinical study done by Minerva in UK, Scientific Reports |8:10474 | DOI:10.1038/s41598-018-28492-w.

(12) Czajka. A. et al., (2018). Daily oral supplementation with collagen peptides combined with vitamins and other bioactive compounds improves skin elasticity and has a beneficial effect on joint and general wellbeing. Clinical study done by Minerva in UK, N U T R I T I O N R E S E A R C H 5 7 ( 2 0 1 8 ) 9 7 – 108.

(13) Slowing down the clock with collagen peptides. Cosmetics Business white paper, January 2021 issue.

(14) Asai TT, Oikawa F, Yoshikawa K, Inoue N, Sato K. (2020) Food-Derived Collagen Peptides, Prolyl-Hydroxyproline (Pro-Hyp), and Hydroxyprolyl-Glycine (Hyp-Gly) Enhance Growth of Primary Cultured Mouse Skin Fibroblast Using Fetal Bovine Serum Free from Hydroxyprolyl Peptide. International Journal of Molecular Sciences. 2020; 21(1):229.

Sports Nutrition

(1) Melvin W. (2005). Dietary Supplements and Sports Performance: Amino Acids. Journal of the International Society of Sports Nutrition. 2: p63-67.

(2) Newholme.E and Co. (1992). Physical and mental fatigue: Metabolic mechanisms and importance of plasma amino acids. British Medical Bulletin, 48: 477-95.

(3) Matsumoto Keitaro and Co. (2008). Branched-Chain Amino acid supplementation increases the lactate threshold during an incremental exercise test in trained individuals. J Nutr Sci Vitaminol.55.p52-58.

(4) Mittleman KD and Co. (1998). Branched-chain amino acids prolong exercise during heat stress in men and women. Med Sci Sports Exerc. 30 (1): 83-91.

(5) Louard RJ and Co. (1990). Effect of infused branched-chain-amino acids on muscle and whole-body amino acid metabolism in man. Clin Sci. 79 (5): 457-466.

(6) Blomstrand E and Co. (2006). Branched-chain-amino acids activate key enzymes in protein synthesis after physical exercise. J Nutr. 136 (I): 269S-73S.

(7) Kimball SR and Co. (2006). Signaling pathways and molecular mechanisms through which branched-chain amino acids mediate translational control of protein synthesis. J Nutr. 136 (I): 227S-31S.

(8) Williams M.H. (2005). Nutrition for health, Fitness & Sports. Boston: McGraw-Hill.

(9) Kim S.K and Co. (2001). Purification and characterization of antioxydative peptides from bovine skin. Journal of Biochemistry and Molecular Biology. Vol 34. 239.

(10) Kjaer M and Co. (2005). Metabolic activity and collagen turnover in human tendon in response to physical activity. J Musculosketet neuronal Interact.5: 41-52.

(11) Langberg H and Co. (1999). Type I collagen synthesis and degradation in peritendinuous tissue after exercise determined by microdialysis in humans. Journal of Physiology.521: 299-306.

(12) Miller B.F and Co. (2005). Coordinated collagen and muscle protein synthesis in human patella tendon and quadriceps muscle after exercise. J Physiol.567 (3). 1021-1033.

(13) Clark, K.L. and Co. (2008). 24-Week study on the use of collagen hydrolysate as a dietary supplement in athletes with activity-related joint pain. Current Medical Research and Opinions, 24 (5): 1485-1496.

(14) Castell LM and Co. (1996). European Journal of Applied Physiology.73: 488-490.

(15) Krieger W James and Co.(2006). Effects of variation in protein and carbohydrate intake on body mass and composition during energy restriction: a meta-regression. Am J Clin Nutr.83:260.74.

(16) Layman K Donald and Co.(2007). Dietary protein and exercise have additive effects on body composition during weight loss in adult women. Journal of Nutrition. 1903-1910.

(17) Faipoux R and Co. (2008). Proteins activate satiety-related neuronal pathways in the brainstem and hypothalamus of rats. The Journal of Nutrition.1172-1178.

(18) Pupovac J and Co. (2002). Dietary peptides induce satiety via cholecystokinin-A and peripheral pioid receptors in rats. The Journal of Nutrition.132: 2775-2780.

(19) Veldhorst M A.B and Co. (2009). A breakfast with alpha-lactalbumin, gelatin, or gelatin+TRP lowers energy intake at lunch compared with a breakfast with casein, soy, whey, or why-GMP. Clinical Nutrition. 28. 147-155.

(20) Hochstenbach-Waelen Ananda and Co. (2009). Single-Protein Casein and Gelatin Diets affect energy expenditure similarly but substrate balance and appetite differently in adults. Journal of Nutrition. 139.p2285-2292.

(21) Manninen AH. (2004). Protein hydrolysates in sports and exercise. Journal of sports science and medicine.3/ 60-63.

(22) Van Loon Luc JC and Co. (2000). Plasma insulin responses after ingestion of different amino acid or protein mixtures with carbohydrate. Am J Clin Nutr. 72: 96-105.

(23) Van Loon Luc JC and Co. (2000). Ingestion of protein hydrolysate and amino acid-carbohydrate mixtures increases postexercise plasma insulin responses in men. American Society of Nutritional Sciences. p2508-2513.

(24) Houston Mark C. (2002). The role of vascular biology, nutrition and nutraceuticals in the prevention and treatment of hypertension. The Journal of the American Nutraceutical Association. Suppl 1.

(25) Herregods Griet. (2011). Activity andpurification of ACE inhibitor peptides from animal by-products: in vitro and in vivo evaluation. PhD thesis. Ghent University, Ghent, Belgium.

(26) Herregods Griet and Co. (2011). Angiotensin I-converting enzyme inhibitor activity of gelatin hydrolysates and identification of bioactive peptides. Journal of agricultural and food chemistry. 59 (2): 552-8.

(27) Clifford, T, et al. (2019): The effects of collagen peptides on muscle damage, inflammation and bone turnover following exercise: a randomized, controlled trial. Amino Acids - https://doi.org/10.1007/s00726-019-02706-5.

(28) Osawa, Y., Nomura, K., Kimira, Y. et al. (2021) Monitoring urinary collagen metabolite changes following collagen peptide ingestion and physical activity using ELISA with anti active collagen oligopeptide antibody. Sci Rep 11, 13527 (2021).